Product: Anti-Caspase-9 (Internal 20 AAs), Polyclonal
Cat. No: PC-018 (100 μg)
Background:
Apoptosis is related to many diseases and
induced by a family of cell death receptors and
their ligands. Cell death signals are transduced
by death domain containing adapter molecules
and members of the caspase family of proteases.
A novel member in the caspase family was
recently identified and designated ICE-LAP6,
Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind
to each other, which leads to caspase-9
activation. Caspase-9 is also activated by
granzyme B and CPP32. Activated caspase-9
cleaves and activates caspase-3 that is one of
the key proteases, being responsible for the
proteolytic cleavage of many key proteins in
apoptosis. Caspase-9 play a central role in cell
death induced by a wide variety of apoptosis
activators including TNF-alpha, TRAIL, anti-CD-
95, FADD, and TRADD. Caspase-9 is
expressed in a variety of human tissues.
Specificity:
Binds caspase-9. It has no cross-response to
other known members of the caspase family.
The immunogen corresponds to amino acids
located within the large subunit of active
caspase-9.
Positive control: Whole cell lysate from HeLa
cells. A 46 kDa band should be detected.
Species Reactivity:
Human, other species not tested.
Ig Isotype:
Rabbit IgG
Immunogen:
A peptide corresponding to amino acids 299 to
318 of human caspase-9.
Format:
Immunoaffinity chromatography purified IgG,
100 μg in 200 μl of PBS containing 0.02%
sodium azide.
Storage and Stability:
Stable for at least one year when stored at 4°C.
Applications and Suggested Dilutions:

Western blot: Suggested dilution: 1:1000 to
1:2000.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.